Friday, May 28, 2010

Breaking Report: Notch Signaling

What's on my desk today? A new report from Saj et al. that combines results from a high-throughput cell-based RNAi screen with in vivo RNAi-based validation.

Saj A, Arziman Z, Stempfle D, van Belle W, Sauder U, Horn T, D├╝rrenberger M, Paro R, Boutros M, Merdes G. A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. Dev Cell. 2010 May 18;18(5):862-76. PubMed PMID: 20493818.

Thursday, May 20, 2010

Breaking Reports: A small-scale and a genome-wide fly RNAi screen

What's on my desk to read today?

A small, focused Drosophila RNAi screen and another that went genome-wide.
Both papers are related to host cell-pathogen interactions.

Serio et al. (2010) Defining a Core Set of Actin Cytoskeletal Proteins Critical for Actin-Based Motility of Rickettsia. Cell Host Microbe 7(5):388-398.

Valanne et al. (2010) Genome-Wide RNA Interference in Drosophila Cells Identifies G Protein-Coupled Receptor Kinase 2 as a Conserved Regulator of NF-{kappa}B Signaling. J Immunol. 2010 Apr 26. [Epub ahead of print].

Wednesday, May 19, 2010

Web Wednesday: E-RNAi

A recent report in NAR describes an update to the E-RNAi tool for dsRNA design.

Horn & Boutros (2010) E-RNAi: a web application for the multi-species design of RNAi reagents—2010 update. Nucleic Acids Research. PMID: 20444868.

Wednesday, May 12, 2010

Web Wednesday: DRSC Gene LookUp ReDesign

We've revamped and added information at our Gene Lookup results page.

Search with a fly gene symbol or other ID, and get a report of what dsRNA amplicons we have for the gene (and if they've hit in screens), what TRiP in vivo RNAi lines we have for the gene, and--scroll to the very bottom--a sense of whether or not the gene is expressed in various cell lines, based on analysis of results from the modENCODE group.

Please check it out! Feedback welcome.

Monday, May 3, 2010

Breaking News: NF-kappa-B Genome-wide Drosophila cell RNAi screen

What's on my desk to read today? A report of a genome-wide fly RNAi screen designed to query the NF kappa B pathway. Includes follow-up studies in zebrafish using morpholinos to knock down gene function in that organism.

Valanne et al. (2010) Genome-Wide RNA Interference in Drosophila Cells Identifies G Protein-Coupled Receptor Kinase 2 as a Conserved Regulator of NF-{kappa}B Signaling. J Immunol. 2010 Apr 26. [Epub ahead of print]. PMID: 20421637.