Friday, March 30, 2012

GenomeRNAi Announces v7.0

The folks at GenomeRNAi just announced release of a version 7.0 of their database, including 28 newly available human cell-based RNAi screen data sets.

Monday, March 26, 2012

Breaking Report: Regulators of Hh Secretion

On my desk to read today? A report on a full-genome screen performed at the DRSC.

The authors include information that might be generally useful to screeners, particularly those performing or currently analyzing the results of luciferase-based assay readouts. The study also includes follow-up using in vivo RNAi.

Aikin et al. (2012) A Genome-Wide RNAi Screen Identifies Regulators of Cholesterol-Modified Hedgehog Secretion in Drosophila. PLoS One. 2012;7(3):e33665. PubMed ID: 22432040

Tuesday, March 20, 2012

FlyMine v33.0

FlyMine recently announced a version 33.0. Details on changes on their blog.

Breaking Report: Proteasome Components & Apical Localization

Also on my desk to read today? A report that includes an in vivo RNAi screen and another that includes screening with deficiencies, classical alleles and in vivo RNAi.

Yano et al. (2012) Identification of proteasome components required for apical localization of Chaoptin using functional genomics. J. Neurogenet. Mar 14. PubMed ID: 22417167

Tan et al. (2012) Genetic screen for regulators of lymph gland homeostasis and hemocyte maturation in Drosophila. G3. 2(3):393-405. PubMed ID: 22413093

Upcoming: Special Issue on RNAi in Genes

The journal Genes is planning a special open access issue on RNA interference.

Guest Editor: Prof. Dr. Jeremy C. Simpson
Deadline for manuscript submissions: 15 August 2012

The Structure of Human Dicer

On my desk to read today? A paper that provides insight into Dicer function.

Lau PW, Guiley KZ, De N, Potter CS, Carragher B, Macrae IJ. The molecular architecture of human Dicer. Nat Struct Mol Biol. 2012 Mar 18. PubMed ID: 22426548

Tuesday, March 13, 2012

FlyBase Survey

Fly people: Take the FlyBase survey! This is our opportunity to be counted as active FlyBase users and make suggestions they will follow up on in future years!

Friday, March 2, 2012

Breaking Report: Cross-system look at the Beta Catenin destruction complex

On my desk to read today? A study that combines cell-based RNAi and in vivo studies in Drosophila with studies in human cultured cells to look at the beta-Catenin destruction complex.

Roberts et al. (2012) Defining components of the bcatenin destruction complex and exploring its regulation and mechanism of action during development. Open Access in PLoS One.