Monday, April 28, 2014

Genome-wide in vivo RNAi screen for genes involved in epithelial development

Berns N, Woichansky I, Friedrichsen S, Kraft N, Riechmann V. A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche. J Cell Sci. 2014 Apr 24. PMID: 24762813.

Tuesday, April 22, 2014

New report on using CRISPR/Cas9 in Drosophila cells

Böttcher R, Hollmann M, Merk K, Nitschko V, Obermaier C, Philippou-Massier J, Wieland I, Gaul U, Förstemann K. Efficient chromosomal gene modification with CRISPR/cas9 and PCR-based homologous recombination donors in cultured Drosophila cells. Nucleic Acids Res. 2014 Apr 19. PMID: 24748663.

From the abstract:  "We present a series of PCR template vectors for C-terminal protein tagging and clonal Drosophila S2 cell lines with stable expression of a myc-tagged cas9 protein." The authors also state that the plasmid constructs associated with the publication will be available at AddGene.

Wednesday, April 9, 2014

Recent review of in vivo RNAi screening

Yamamoto-Hino M, Goto S. In Vivo RNAi-Based Screens: Studies in Model Organisms. Genes (Basel). 2013 Nov 25;4(4):646-65. PMID: 24705267.

Tuesday, April 8, 2014

in vivo screen of about 300 genes related to the 'periodic transcriptome'

Liang L, Haug JS, Seidel CW, Gibson MC. Functional Genomic Analysis of the Periodic Transcriptome in the Developing Drosophila Wing. Dev Cell. 2014 Mar 27. pii: S1534-5807(14)00134-8. PMID: 24684830.

Spindle assembly screen of 96 candidate genes

Gallaud E, Caous R, Pascal A, Bazile F, Gagné JP, Huet S, Poirier GG, Chrétien D, Richard-Parpaillon L, Giet R. Ensconsin/Map7 promotes microtubule growth and centrosome separation in Drosophila neural stem cells. J Cell Biol. 2014 Mar 31;204(7):1111-21. PMID: 24687279.

From the abstract: "... To identify new mitotic spindle assembly regulators, we isolated 855 microtubule-associated proteins (MAPs) from Drosophila melanogaster mitotic or interphasic embryos. Using RNAi, we screened 96 poorly characterized genes in the Drosophila central nervous system to establish their possible role during spindle assembly. ..."

Review--RNAi screening and signaling networks

Evans L, Sailem H, Vargas PP, Bakal C. Inferring signalling networks from images. J Microsc. 2013 Oct;252(1):1-7. PMID: 23841886.

From the abstract:  "This review describes the methodology used to map signalling networks using data generated in the context of RNAi screens."

Tuesday, April 1, 2014

Detailed methods report for TALEN-based genome engineering in flies

Zhang X, Ferreira IR, Schnorrer F. A simple TALEN-based protocol for efficient genome-editing in Drosophila. Methods. 2014 Mar 26. pii: S1046-2023(14)00122-4. PMID: 24680697.