Tuesday, March 17, 2015

Review of fly CRISPR approaches

Jiang Xua, Xingjie Rena, Jin Suna, Xia Wanga, Huan-Huan Qiaoa, Bo-Wen Xua, Lu-Ping Liua,  Jian-Quan Ni (2014) A Toolkit of CRISPR-Based Genome Editing Systems in Drosophila. In press manuscript available at Journal of Genetics and Genomics

From the abstract: "... we focus on the applications of this popular system in Drosophila. We discuss the effectiveness of the CRISPR/Cas9 systems in terms of delivery, mutagenesis detection, parameters affecting efficiency, and off-target issues, with an emphasis on how to apply this powerful tool to characterize gene functions."

See also:  igtrcn.org/pulling-recent-crisprcas9-work-in-drosophila-together/

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