Wednesday, April 8, 2015

Report of improved CRISPR-based transcriptional activation approach includes work in Drosophila cultured cells

Chavez A, Scheiman J, Vora S, Pruitt BW, Tuttle M, P R Iyer E, Lin S, Kiani S, Guzman CD, Wiegand DJ, Ter-Ovanesyan D, Braff JL, Davidsohn N, Housden BE, Perrimon N, Weiss R, Aach J, Collins JJ, Church GM. Highly efficient Cas9-mediated transcriptional programming. Nat Methods. 2015 Apr;12(4):326-8. PMID: 25730490.

From the abstract: "... modest levels of gene activation have limited potential applications. We describe an improved transcriptional regulator obtained through the rational design of a tripartite activator, VP64-p65-Rta (VPR), fused to nuclease-null Cas9. ..."

Drosophila S2R+ cultured cells are among the systems in which this approach was successfully tested. See Results and Supplementary Fig. 9.

No comments: